RESUMO
Sera from 282 equines from Tandil country and surroundings were investigated searching for hemagglutination inhibition (HI), Complement fixation (CF), and Neutralizing (NT) antibodies against three flavivirus:Ilheus, St. Louis Encephalitis, and Yellow Fever from the Togaviridae family. Sera were collected between 3-20-79 and 11-25-80 from 10 different places in Tandil and Ayacucho countries. Animals ranged from 45 days to 27 years old. Forty nine of them reacted with one or more flavivirus by HI and/or CF tes representing a prevalence of 17.4% for this antigenic complex. Twenty four of them neutralized ILH, 6 SLE and from them 2 neutralized both viruses. Twenty one cannot be endorsed to ILH or SLE but to some other/s flavivirus present in the environment but not present in the test since they did not neutralize any of them. Other 12 sera with no HI or CF antibodies for any of the three viruses did not neutralize ILH or SLE. Results showed ILH and SLE viruses, both isolated in man during previous years studied in Argentina, to be endemic in the area and to be naturally infecting horses, perhaps without producing detectable disease, since no epizootics due to SLE or ILH viruses were reported on the horse population investigated.
Assuntos
Flavivirus/isolamento & purificação , Doenças dos Cavalos/microbiologia , Cavalos/microbiologia , Infecções por Togaviridae/veterinária , Animais , Anticorpos Antivirais/análise , Argentina , Reservatórios de Doenças , Flavivirus/imunologia , Doenças dos Cavalos/epidemiologia , Infecções por Togaviridae/epidemiologia , Infecções por Togaviridae/microbiologiaRESUMO
Sera from 282 equines from Tandil country and surroundings were investigated searching for hemagglutination inhibition (HI), Complement fixation (CF), and Neutralizing (NT) antibodies against three flavivirus:Ilheus, St. Louis Encephalitis, and Yellow Fever from the Togaviridae family. Sera were collected between 3-20-79 and 11-25-80 from 10 different places in Tandil and Ayacucho countries. Animals ranged from 45 days to 27 years old. Forty nine of them reacted with one or more flavivirus by HI and/or CF tes representing a prevalence of 17.4
for this antigenic complex. Twenty four of them neutralized ILH, 6 SLE and from them 2 neutralized both viruses. Twenty one cannot be endorsed to ILH or SLE but to some other/s flavivirus present in the environment but not present in the test since they did not neutralize any of them. Other 12 sera with no HI or CF antibodies for any of the three viruses did not neutralize ILH or SLE. Results showed ILH and SLE viruses, both isolated in man during previous years studied in Argentina, to be endemic in the area and to be naturally infecting horses, perhaps without producing detectable disease, since no epizootics due to SLE or ILH viruses were reported on the horse population investigated.
RESUMO
Se describe la patologia pulmonar presentada por cobayos inoculados con la cepa Portillo (Noguera) de virus Junin, aislada de un caso de sindrome uremico hemolitico (SUH) atendido en el Hospital de Ninos de Buenos Aires (5). Macroscopicamente hubo cambio de coloracion en algunos lobulos pulmonares principalmente los diagramaticos, microscopicamente se observo siempre una neumonitis proliferativa distribuida en focos con presencia de elementos linfoides, macrofagos y celulas de las paredes alveolares que consolidaban el parenquima pulmonar. Se destaca la habilidad de la cepa aislada de SUH de producir lesiones pulmonares experimentales en cobayos y idiosincracia del sindrome en ninos en donde uno de los cuadros previos a la crisis hemolitica observada es una neumopati
Assuntos
Animais , Camundongos , Pneumopatias , Síndrome Hemolítico-Urêmica , Arenavirus do Novo MundoRESUMO
Se describe la patologia pulmonar presentada por cobayos inoculados con la cepa Portillo (Noguera) de virus Junin, aislada de un caso de sindrome uremico hemolitico (SUH) atendido en el Hospital de Ninos de Buenos Aires (5). Macroscopicamente hubo cambio de coloracion en algunos lobulos pulmonares principalmente los diagramaticos, microscopicamente se observo siempre una neumonitis proliferativa distribuida en focos con presencia de elementos linfoides, macrofagos y celulas de las paredes alveolares que consolidaban el parenquima pulmonar. Se destaca la habilidad de la cepa aislada de SUH de producir lesiones pulmonares experimentales en cobayos y idiosincracia del sindrome en ninos en donde uno de los cuadros previos a la crisis hemolitica observada es una neumopati
Assuntos
Animais , Camundongos , Arenavirus do Novo Mundo , Síndrome Hemolítico-Urêmica , PneumopatiasRESUMO
Se analizaron 336 sueros humanos obtenidos entre el 24-4-79 y el 26-11-81, del partido de Tandil, para determinar la presencia o no en esta latitud de 3 Flavivirus conocidos para la Republica Argentina. Se pudo establecer las actividades de los virus encefalitis de San Luis e Ilheus.No ha habido actividad de fiebre amarilla a pesar de que algunos de los individuos encuestados tienen mas de 80 anos. Se admite la existencia a otro/s Flavivirus aun no identificado/s, diferente a los dos (SLE e ILH) ya constatados como endemicos
Assuntos
Pré-Escolar , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Humanos , Masculino , Feminino , Flavivirus , Infecções por Togaviridae , ArgentinaRESUMO
Se analizaron 336 sueros humanos obtenidos entre el 24-4-79 y el 26-11-81, del partido de Tandil, para determinar la presencia o no en esta latitud de 3 Flavivirus conocidos para la Republica Argentina. Se pudo establecer las actividades de los virus encefalitis de San Luis e Ilheus.No ha habido actividad de fiebre amarilla a pesar de que algunos de los individuos encuestados tienen mas de 80 anos. Se admite la existencia a otro/s Flavivirus aun no identificado/s, diferente a los dos (SLE e ILH) ya constatados como endemicos
Assuntos
Pré-Escolar , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Humanos , Masculino , Feminino , Flavivirus , Infecções por Togaviridae , ArgentinaRESUMO
Ehrlich ascitic carcinoma, as developed in albino mice, has been used as a source of hemagglutinating and complement-fixing antigens, and it proved to be suitable for one type of antigen, or both, for at least 12 viruses of 16 tested. Hemagglutinins were obtained with members of arbovirus groups A, B, and C; complement-fixing antigens were obtained for at least one member of each antigenic group tested. Ehrlich ascitic tumor was compared with sarcoma 180 as a source of antigens; although sarcoma 180 showed many advantages over Ehrlich tumor, the latter gave, in general, better results for complement-fixing antigens. Oncolytic effect with complete recovery of the mice was observed in some instances. The highest recovery rate resulted with Congo and UNA viruses (40%), and the second highest rate resulted with dengue 2, St. Louis, Hazara, and Uukuniemi viruses (20%). The best survival was observed, in decreasing order, with Congo, St. Louis, dengue 2, Tacaribe, Sindbis, Junin, and Amapari viruses.
Assuntos
Antígenos Virais , Carcinoma de Ehrlich/microbiologia , Hemaglutininas Virais , Fenômenos Fisiológicos Virais , Animais , Bunyaviridae/fisiologia , Carcinoma de Ehrlich/imunologia , Carcinoma de Ehrlich/patologia , Testes de Fixação de Complemento , Camundongos , Reoviridae/fisiologia , Sarcoma 180/microbiologia , Togaviridae/fisiologiaRESUMO
Se relata el primer aislamiento de virus Ilheus en Argentinas, hecho en 1963 de una paciente con enfermedad febril sistemica que mostro seroconversion para algunos flavivirus por inhibicion de la hemoaglutinacion, con titulos maximos para Ilheus La cepa fue aislada de suero tomado al tercer dia del comienzo de la enfermedad usando como huespedes ratones de 0-2 dias de edad. El virus aislado mostro ser de grupo B por fijacion de complemento. Y por pruebas de neutralizacion cruzada fue indiferenciable de la cepa prototipo de virus Ilheus
Assuntos
Adulto , Humanos , Feminino , Flavivirus , Testes de Fixação de Complemento , Testes de Inibição da Hemaglutinação , ArgentinaRESUMO
Se relata el primer aislamiento de virus Ilheus en Argentinas, hecho en 1963 de una paciente con enfermedad febril sistemica que mostro seroconversion para algunos flavivirus por inhibicion de la hemoaglutinacion, con titulos maximos para Ilheus La cepa fue aislada de suero tomado al tercer dia del comienzo de la enfermedad usando como huespedes ratones de 0-2 dias de edad. El virus aislado mostro ser de grupo B por fijacion de complemento. Y por pruebas de neutralizacion cruzada fue indiferenciable de la cepa prototipo de virus Ilheus
Assuntos
Adulto , Humanos , Feminino , Testes de Fixação de Complemento , Flavivirus , Testes de Inibição da Hemaglutinação , ArgentinaAssuntos
Formação de Anticorpos , Febres Hemorrágicas Virais/imunologia , Paralisia/microbiologia , Vírus de RNA/imunologia , Animais , Animais Recém-Nascidos , Anticorpos Antivirais/análise , Encéfalo , Testes de Fixação de Complemento , Reações Cruzadas , Diagnóstico Diferencial , Febres Hemorrágicas Virais/diagnóstico , Febres Hemorrágicas Virais/microbiologia , Injeções , Injeções Intraperitoneais , Injeções Subcutâneas , Camundongos , Testes de Neutralização , Fatores de TempoRESUMO
Antigens for Grand Arbaud, Hazara, and California arboviruses were able to agglutinate goose and either dog, hamster and guinea pig, or hamster red blood cells (RBC) to the same titer at the same pH; in hemagglutination-inhibition (HI) tests, titers for homologous and related sera were the same with these different types of RBC or occasionally one dilution higher with the mammalian cells. Antigens for St. Louis encephalitis and Eastern equine encephalitis viruses required use of lower antigen dilutions with human, guinea pig, and hamster RBC than with goose RBC. The results of comparative HI testing with these latter antigens and types of RBC indicate that HI titer is not directly related to the antigen dilution used with different types of RBC.
